Metabolic profiling and biological activity of two Livistona species: L. chinensis and L. australis

Livistona is a genus of family Arecaceae and widely grown in tropical areas. The phytochemical analysis of the leaves and fruits of two Livistona species, L. chinensis and L. australis were carried out using UPLC/MS and determination of the total phenolic and total flavonoid contents, in addition to the isolation and identification of five phenolic compounds and one fatty acid from L. australis fruits. The total phenolic compounds varied from 19.72 to 78.87 mg GAE g−1 dry plant, while the total flavonoid contents were in the range of 4.82–17.75 mg RE g−1 dry plant. The UPLC/MS analysis of the two species led to the characterization of forty-four metabolites belonging mainly to the different classes of flavonoids and phenolic acids, while the compounds isolated from L. australis fruits were identified as gallic acid, vanillic acid, protocatechuic acid, hyperoside, quercetin 3-O-α-d-arabinopyranoside and dodecanoic acid. The in vitro biological evaluation of L. australis leaves and fruits were estimated as anticholinesterase, telomerase reverse transcriptase (TERT) potentiation and anti-diabetic through measuring the capacity of the extracts to inhibit dipeptidyl peptidase (DPP-IV). The results revealed that the leaves showed remarkable anticholinesterase and antidiabetic activity compared to fruits with IC50 values of 65.55 ± 3.75 ng mL−1 and 90.8 ± 4.48 ng mL−1, respectively. In the TERT enzyme assay, the leaves extract triggered a 1.49-fold increase in telomerase activity. This work showed that the Livistona species are a good source for flavonoids and phenolics, which play an important role in anti-aging and the treatment of chronic diseases, such as diabetes and Alzheimer's.


Introduction
Alzheimer's disease (AD) is a neurodegenerative disorder and is considered to be the main cause of dementia. Worldwide, about 40 million people are expected to suffer from dementia, and this number is expected to be doubled by 2050. 1 A low level of acetylcholine in the brain is the key marker of the disease. Inhibition of the acetylcholinesterase (AChE) enzyme, which is responsible for the degradation of acetylcholine, is the most promising approach for the treatment of AD. Many natural compounds, such as terpenoids, coumarins, avonoids, alkaloids and other phenolic compounds, have shown anticholinesterase activity. 2 Another sign associated with aging is telomere attrition. At the ends of linear chromosomes are DNA repeats called telomeres, which ensure chromosome stability throughout replication. Every time a cell divides, as well as when there is oxidative stress, the telomere length is decreased. When telomeres signicantly shorten in length, cells can no longer divide, causing cell death. There is an enzyme called telomerase, which creates the particular DNA sequence at the telomeres. [3][4][5][6][7] The substances that potentiate telomerase are called telomerase activators and are used as antiageing sources.
Another heath problem that has an impact on human health is T2DM. The number of patients expected to suffer from diabetes in 2040 will be 642 million worldwide, according to the International Diabetes Federation. 8 Lowering the blood glucose level is the primary treatment of T2DM, which can be done through a-glucosidase inhibitors or the stimulation of insulin secretion. 9 Incretins are hormones that are released from the small intestine in response to dietary intake, and have an impact similar to glucagon. The main incretins are a glucagonlike peptide (GLP-1) and a glucose-dependent insulinotropic peptide (GIP). These incretins potentiate insulin secretion from pancreatic B-cells. 10 Dipeptidyl peptidase-IV is responsible for the degradation of these incretins. Thus, DPP-IV inhibitors prevent the degradation of these incretins, which led to an increase in the insulin level and lowering of the blood glucose level. 11 Livistona is a genus belonging to the family Arecaceae and it is widely distributed in tropical areas, with about 36 species reported in the genus. Phytochemical studies on Livistona have reported on many compounds, such as avonoids, phenolics, ceramides and glycerides, [12][13][14] pyranone derivatives 15 and sulfated avonoids. 16 Biologically, L. chinensis has been reported to have anticancer 17 and cardioprotective 18 properties, while L. decipiens has been reported to have antioxidant and cytotoxic activities. 19 A recent report on the leaves and fruit extracts of the plant also showed inhibitory activity against coronavirus. 20 The fruits of L. chinensis are edible in eastern Asia, they are a component of prepared soups used in the treatment of liver cancer and chronic hepatitis. 21 The nutritional evaluation of the leaves and fruits of L. australis also showed that the fruits are a good source of calcium and potassium. 22 This work aims to identify the secondary metabolites in the leaves and fruits of L. chinensis and L. australis using UPLC coupled with high-resolution electron spray ionization mass (HRESIMS), and evaluate their anticholinesterase, DPP-IV inhibitory potential, and telomerase activation potential.

Plant material
The fruits and leaves of Livistona australis and Livistona chinensis were supplied from El-Orman Botanical Garden, Giza, Egypt on Jan. 2018. The samples were authenticated by Eng. Trease Labib (El Orman Botanical Garden) and Dr Reem Samir Hamdy (Plant Taxonomy, Botany Department, Faculty of Science, Cairo University, Giza, Egypt). Voucher specimens (BUPD-71-2018 for L. australis and BUPD-73-2018 for L. chinensis) were kept in the Pharmacognosy Department, Faculty of Pharmacy, Beni-Suef University, Egypt. The plants were cleaned, dried and nely powdered for the study.

Apparatus
The NMR analysis for the isolated compounds was carried out using Bruker Avance III 400 MHz (Bruker, USA). LC/MS analysis of the extracts was done on an Acquity Ultra Performance Liquid Chromatography (UPLC) system connected with a Synapt G2 HDMS quadrupole time-of-ight hybrid mass spectrometer (Waters, USA). Spectrophotometric determination of the total phenolic and avonoid contents was measured using a Jenway 6305 spectrophotometer.

Metabolomics study
Three grams of each sample were exhaustively defatted using nhexane (3 × 15 mL). The marc was then extracted with 70% ethanol, ltered with Whatman lter paper, and evaporated under vacuum to afford the dry residue. One mg of each extract was dissolved in 1 mL methanol and analyzed using LC/MS, as reported by Owis et al., 2020. 23 Briey, the samples were analyzed using a C-18 column (2.1 × 100 mm, 1.7 mm particle size; Waters, Milford, MA, USA). A binary solvent was used for separation; solvent A was 0.1% formic acid in water, while solvent B was acetonitrile, and a gradient elution from 0-100% of solvent B in 6 min with ow rate 0.3 mL min −1 was followed. Electron spray ionization mass spectrometry was used to detect the metabolites; the processed data set was subjected to molecular formula prediction, as well as peak identication. The positive and negative ionization mode data sets from the plant extract were dereplicated against the Dictionary of Natural Products (DNP) database.

Determination of total phenolics and total avonoids contents
The fruits and leaves of L. australis and L. chinensis at 1 g each were extracted with 50 mL of 80% methanol for 2 h on an orbital shaker set at 200 rpm. Each mixture was separately centrifuged for 20 min, and the supernatants were decanted. The residues were re-extracted under the same conditions. Supernatants were separately combined. The volume was adjusted to 100 mL; these extracts were used for total phenolic and total avonoid determination. The total phenolic content was assessed using Folin-Ciocalteu reagent as follows. 24 A volume of 300 mL of each extract was mixed with 2.25 mL Folin reagent. Aer 5 minutes, 2.25 mL of 6% sodium carbonate was added. The mixture was rested for 90 min, and the blue color that developed was measured at 725 nm. The results were expressed as mg gallic acid equivalents per g dried sample (mg GAE g −1 ). Meanwhile, the avonoid content was assessed by colorimetric method. 24 Briey, 0.5 mL of each extract was diluted with 2.25 mL distilled water, and then 150 mL of 5% NaNO 2 was added. Six min later, 300 mL of 10% AlCl 3 was mixed and the reaction was rested for 6 min. Finally, 1 mL of 1 M NaOH was added. The mixture was mixed well and the absorbance was measured at 510 nm. Results of the total avonoids were expressed as mg rutin equivalents per g of dried sample (mg RE g −1 ).

Extraction and fractionation
Air-dried, powdered pericarp of L. australis (1.75 kg) was extracted with 70% ethanol (5 L) by cold maceration for 72 h, and then ltered through lter paper. The marc was re-extracted again until exhaustion, and the collected extracts was dried under vacuum at 45°C to afford the crude extract (340 g). A part of the dried extract (200 g) was fractionated using n-hexane, dichloromethane and ethyl acetate to give 22, 14 and 6 g, respectively. The n-hexane fraction (6 g) was chromatographed on a silica gel (E-Merck, Germany) column using hexane with 10% ethyl acetate increments to afford one major compound 1 (500 mg). The ethyl acetate fraction (5 g) was chromatographed on a polyamide (E-Merck, Germany) column starting with 100% water as the eluent, then with decreasing polarity by 20% MeOH to afford ve sub-fractions. The sub-fraction eluted with 20% water (500 mg) was re-chromatographed on a Sephadex LH-20 column using methanol to afford compounds 2 and 3 (25, 10 mg, respectively). The sub-fraction eluted with 60% water (100 mg) was re-chromatographed on a Sephadex LH-20 column (Sigma-Aldrich, USA) using methanol as an eluent to afford three compounds 4-6 (15, 12, 20 mg, respectively).

2.7.
Biological studies 2.7.1. Anticholinesterase activity. The anticholinesterase potential of L. australis leaves and fruits extracts, as well as donepezil standard, were measured utilizing Ellman's method. 25 The method depends on the cleavage of acetylthiocholine substrate into thiocholine by the acetylcholinesterase enzyme. Thiocholine reacts with Ellman's reagent [5,5 ′dithiobis(2-nitrobenzoic acid), known as DTNB], to give 2nitrobenzoate-5-mercaptothiocholine and 5-thio-2nitrobenzoate, which can be detected at 412 nm. The experiment was done according to the manufacturer's instructions in triplicate, and the IC 50 values for the tested extracts were calculated as follows; a mixture of 25 mL of acetylthiocholine iodide (5 mM), 125 mL of DTNB (3 mM), 50 mL of buffer B (50 mM Tris-HCl, pH 8, 0.1% BSA) and 25 mL of each test extract solution at different concentrations were added. The mixture was then incubated for 10 min at 37°C, then 25 mL of AChE (0.05 U mL −1 ) was added to start the reaction. A negative control (25% DMSO in MeOH) was used.
2.7.2. Antiaging activity. In vitro assay to measure TERT in response to the tested extracts and curcumin standard was carried out using the human broblast cell line (HFB4), which is known as the normal human skin melanocyte cell line. The assay was carried out according to the method reported by Refaey, Abdelhamid et al. 2021. 26 HFB4 was obtained from (VACSERA, Giza, Egypt) and cultured in RPMI-1640 media supplemented with 10% heat-inactivated FBS, 100 U mL −1 penicillin, and 100 U mL −1 streptomycin. In a six well plate, the cells were plated with a density of 2 × 10 5 cells per well and incubated at 37°C for 24 h in a humid atmosphere of 5% CO 2 . The tested extracts and curcumin (positive control) were added to the cells. Aer 24 h, the supernatant was collected and centrifuged for 20 min, which was then used to carry out the assay. The collected supernatants were added to precoated microtiter plates with a biotin-detection antibody specic to TERT, then streptavidin conjugated to horse-radish peroxidase (SABC) was added to each microplate well and incubated. The color of the solutions in the wells containing TERT, the antibody and enzyme-conjugated streptavidin will change aer the addition of 3,3 ′ ,5,5 ′ -tetramethylbenzidine (TMB) substrate solution. The stop solution was used to stop the enzymesubstrate reaction, and the color change at 450 nm was determined spectrophotometrically. The optical density of the samples was then compared to the standard curve in order to determine the TERT concentration in the samples.
2.7.3. Antidiabetic activity. The antidiabetic potential of the leaves and fruits extract was assessed by measuring the ability of the extract to inhibit DPP-IV using the DPP-IV inhibition screening kit (Catalog Number KA3738, Abnova, Taiwan). The assay depends on the ability of DPP-IV to break down a uorogenic substrate, Gly-Pro-Aminomethylcoumarin, and release products measured by uorometer (E m /E x = 360/460 nm). In the presence of a DPP-IV inhibitor, the cleavage will be inhibited. The procedure of the experiment was done according to the supplier instructions, and as reported by Kempegowda, Zameer et al. 2018. 27 Different concentrations of the tested extracts were dissolved in DMSO. A volume of 10 mL of the sample solution was mixed with 30 mL assay buffer and 10 mL enzyme solution in a 96-well plate, then 50 mL of a diluted substrate solution was added to start the reaction and incubated at 37°C for 30 min. Fluorescence with an excitation wavelength of 360 nm and an emission wavelength of 460 nm was monitored using a plate reader. The IC 50 for the tested extracts and sitagliptin was calculated.

Statistical analysis
Data were presented as the mean ± standard deviation (SD). All analyses were done in triplicate. Statistical and graphical evaluations were done using Graph Pad Prism 7 and Microso Excel 2010.

Metabolic analysis
Metabolic proling of the secondary metabolites in L. chinensis and L. australis leaves and fruits were conducted using LC-HRESIMS (chromatograms in ESI data, Fig. S1-S8 †) for dereplication/detection of the putative compounds responsible for the various activities assigned to Livistona. It led to the characterization of forty-four compounds ( Table 1, Fig. 1) belonging to different classes of avonoids and phenolic acids.
3.1.1. Phenolic acids. Twelve phenolic acids (six hydroxybenzoic acids and six hydroxycinnamic acids) were annotated in the extracts of the leaves and fruits of L. chinensis and L. australis. The hydroxybenzoic acids were identied as syringic acid, 4-hydroxy benzoic acid, procatechuic acid, vanillic acid, isovanillic acid and homovanillic acid. They were characterized from the mass ion peaks at m/z 197.0447, 137.0238, 153.0189, 167.0342, 181.0498 and molecular formulas C 9 H 10 O 5, C 7 H 6 O 3, C 7 H 6 O 4 , C 8 H 8 O 4 and C 9 H 10 O 4 , respectively. Syringic acid, procatechuic acid, vanillic acid, isovanillic acid and homovanillic were detected in the leaves and fruit extracts of L. chinensis and L. australis, while 4-hydroxy benzoic acid was detected only in the fruit extract of both plants. These phenolic acids were detected previously in L. chinensis leaves. 28 A metabolite with a mass ion peak at m/z 353.0881 and molecular formula of C 16 H 18 O 9 was detected in the leaves and fruit extracts of both plants, and was annotated as chlorogenic acid or its isomers cryptochlorogenic acid or neochlorogenic acid, which were previously detected in L. chinensis leaves. 28 Also caffeic acid was recognized in all analyzed extracts from the mass ion peak at m/ z 179.0346 and the molecular formula C 9 H 8 O 4 . 5-O-caffeoylshikimic acid or 3-O-caffeoylshikimic acid was characterized from the mass ion peak at m/z 335.0769 and molecular formula C 16 H 16 O 8 in the fruit and leaves of L. chinensis and in the fruit extract of L. australis. Caffeic acid and caffeoylshikimic acid were previously reported in L. chinensis fruits. 29 3.1.2. Flavonoids. Thirty-two avonoids were characterized in the leaves and fruit extracts of L. chinensis and L. australis. The metabolite with the mass ion peak at m/z 609.1455, in accordance with the molecular formula C 27 H 30 O 16 , was detected in the L. australis fruit extract and recognized as rutin, which was formerly isolated from the L. chinensis fruit extract. 30 Another metabolite was detected in the L. chinensis leaves and fruit with molecular ion peak at m/z 593.1514, which is in agreement with the molecular formula C 27 H 30 O 15 and was annotated as vienin II. This was previously reported in L. decipiens leaves. 19 Moreover, the metabolites with the mass ion vitexin 7-O-sulfate (or its isomer isovitexin 7-O-sulfate), respectively. These metabolites were detected in L. chinensis leaves and fruits and L. australis leaves, and were previously reported in L. chinensis leaves. 28 The mass ion peaks at m/z 447.0931 and 431.0974 with molecular formulas of C 21 H 20 O 11 and C 21 H 20 O 10 were identied as orientin (or its isomer isoorientin) and vitexin (or isovitexin), which are C-glycosyl avones.
Orientin was detected in all tested extracts, while vitexin was detected in L. chinensis leaves and fruits. Both compounds were previously reported in L. chinensis leaves. 18 Another metabolite was detected in the leaves and fruits of L. chinensis and L. australis with a molecular ion peak at m/z 447.0932, and was in agreement with the molecular formula C 21 H 20 O 11 . It was identied as cynaroside (luteolin 7-glucoside) and previously reported in L. chinensis leaves. 28 The metabolite with mass ion peak at m/z 563.1405 in agreement with the molecular formula C 26 H 28 O 14 was identied as schaoside (or isoschaoside). The metabolite was detected in the leaves and fruits of L. chinensis and L. australis leaves, and was previously reported in L. chinensis leaves. 28 The metabolite with mass ion peak at m/z 289.0713 in accordance with the molecular formula C 15 H 14 O 6 was identied as catechin (avan-3-ol). The compound was detected in the leaves and fruits of L. australis and L. chinensis fruits. It was previously reported in L. chinensis fruits. 30  Quercitrin was detected in all tested extracts, and previously reported in L. chinensis fruits. 30 Meanwhile, hyperoside and isoquercitrin were detected in L. chinensis fruits, L. australis leaves and fruits, they were previously reported in L. australis leaves. 16 Quercetin and quercetin 3-arabinoside were detected in L. australis fruits and previously reported in L. australis leaves and Serenoa repens fruit, respectively. 16,31 The metabolite with mass ion peak at 571.0759 in agreement with the molecular formula C 23 H 24 O 15 S was identied as tricin 7-O-b-glucopyranoside-2 ′′ -sulphate sodium salt, which was detected in L. chinensis and L. australis leaves and previously reported in L. australis leaves. 16 The mass ion peak at m/z 477.1032 and molecular formula C 22 H 22 O 12 was detected in the fruits of L. chinensis and L. australis, identied as isorhamnetin-3-O-b-D-glucopyranoside, and was previously reported in L. chinensis fruits. 30 The metabolite with mass ion peak at m/z 273.0766 and molecular formula C 15 H 14 O 5 was characterized as 3,5,3 ′ ,5 ′ -tetrahydroxy-4 ′ -methoxystilbene or (−)-epiafzelechin. The metabolite was detected in the fruit extracts of both species, and previously reported in L. chinensis fruits. 13 The metabolites with mass ion peaks at 285.  australis leaves, while 7-O-methylluteolin and tricin were reported in L. chinensis. 16,18,28,32 3.2. Total phenolic and total avonoids content Folin-Ciocalteu reagent was used to determine the total phenolic content in the fruits and leaves of L. australis and L. chinensis extracts. The Folin-Ciocalteu reagent consists of complex polymeric ions (yellow solution) formed from phosphotungstic and phosphomolybdic heteropolyacids. This reagent produces complex molybdenum-tungsten blue from the oxidation of phenolates, which can be spectrophotometrically detected at 725 nm. 24 As shown in Table 2, the order of the phenolic content was L. australis leaves > L. chinensis fruit > L. australis fruits > L. chinensis leaves with the values of 78.87, 44.50, 32.82 and 19.72 mg GAE g −1 , respectively. Flavonoids are the widely distributed and most common group of plant phenolics, which are characterized by the presence of a benzo-g-pyrone structure. 33 The total avonoids content was determined in the assigned extracts by reaction with sodium nitrite, followed by the formation of a colored complex of avonoid-aluminum using aluminum chloride that was spectrophotometrically monitored at 510 nm. 24 For both Livistona species, the samples containing the highest avonoid content was L. chinensis fruit (17.75 mg RE g −1 ), followed by the L. australis fruit (12.81 mg RE g −1 ), L. australis leaves (8.16 mg RE g −1 ) and L. chinensis leaves (4.82 mg RE g −1 ), as indicated in Table 2.
The total avonoid and phenolic contents for L. chinensis previously reported and the results were found to be 139.51 ± 5.90 mg g −1 dry plant and 112.07 ± 2.24 mg g −1 dry plant, respectively 28 while the L. speciosa seed extract showed a total phenolic content of 2.35 mg of gallic acid/1 g of sample, and the total avonoid was found 39.27 mg of rutin/1 g of sample. 34 In our study, the L. australis leaves had the highest phenolic content, while the L. chinensis fruits showed a high avonoid content.

Identication of the isolated compounds
Six known compounds were isolated and identied from the pericarp of L. australis: one fatty acid from the hexane fraction and ve phenolic compounds from the ethyl acetate fraction. The chemical structures of the pure isolated compounds were elucidated using NMR spectrometry, and conrmed by comparing the obtained data with those previously reported in the literature (Fig. 2). The identied compounds were: (1) dodecanoic acid; (lauric acid,C 12 H 24 O 2 ), 35 (2) (6) procatechuic acid (C 7 H 6 O 4 ). 39 These compounds have been isolated and characterized for the rst time from L. australis fruits (spectroscopic data in ESI data, Fig. S9-S20 †).

Biological studies
Phenolic compounds have been reported to lower the incidence of many chronic diseases, such as cancer, cardiovascular diseases (CVD), chronic respiratory diseases, diabetes and neurodegenerative diseases. 40 In this work, extracts of L. australis showed a higher content of phenolic compounds than L. chinensis, so the biological evaluation was done for L. australis regarding anticholinesterase, and antiaging and antidiabetic properties.
3.4.1. Anticholinesterase activity. A key neurotransmitter in the pathogenesis of Alzheimer's disease (AD) is acetylcholine, which is hydrolyzed by acetyl-cholinesterase (AChE) and butyrylcholinesterase (BuChE). AD is associated with low level of acetylcholine in specic regions of the brain. 41,42 AChE inhibitors, which led to high levels of acetylcholine in the brain, are considered to be the most effective strategy in AD treatment. 43,44 Extracts of L. australis leaves and fruits were screened for AChE inhibitory activity using Ellman's spectrophotometric method. The results showed that the inhibitory concentration (IC 50 ) was 65.55 ± 3.75 ng mL −1 and 122 ± 6.97 ng mL −1 for leaves and fruits, respectively, compared with donepezil as a standard with (IC 50 ) at 49.44 ± 2.82 ng mL −1 . The results (Table 3) revealed that the leaves extract was active as an antiacetylcholinesterase compared with the standard. Flavonoids are a promising class of natural compounds in the management of AD disease. 45 A previous report on the anticholinesterase activity of Sabal blackburniana (Palmae) was performed, and the results showed a good activity of avonoid aglycone against the acetylcholinesterase enzyme, such as luteolin, taxifolin, tricin, genistein, chrysoeriol and catechin. 46 L. australis leaves and fruit extracts contained many avonoid aglycones, such as catechin, luteolin and tricin. Another report revealed that apigenin, vanillic acid and acacetin-7-O-b-D-glucoside showed potent anticholinesterase activity. 26 Finally, it can be concluded that the anti-cholinesterase activity may refer to avonoids and phenolic acids detected in the analyzed extracts. a Values are presented in mean ± SD (n = 3), expressed as mg gallic acid equivalent per g of dry sample (mg GAE g −1 ) for total phenolics and as mg rutin equivalent per g of dry sample (mg RE g −1 ) for total avonoids. 3.4.2. Antiaging activity. Telomere damage is one of the primary causes of aging. It is noteworthy that many chronic diseases, such as cancer, heart diseases, diabetes, and kidney diseases, are related to ageing. 7 In human cells, telomerase expression and activity decline with ageing. 47 Gene therapy with hTERT or telomerase activators are the strategies commonly used for treatment of telomerase-dependent disease. However, gene therapy is not a practical medical intervention, so the attractive alternative would be a chemical telomerase activator, which would allow for a more precise control over the dose and timing. 48 Previous studies on the root extract of Astragalus membranaceus led to an increase in the concentration of telomerase enzyme in the tested cells. 49 Our results (Table 3) revealed that L. australis leaves were a potent extract as it caused a 1.49-fold increase in the telomerase activity, followed by L. australis fruit extract with a 1.41-fold increase in the telomerase activity. Meanwhile, curcumin as the standard caused a 1.54fold increase in telomerase activity.
3.4.3. Antidiabetic activity. DPP-IV is a serine protease that cleaves N-terminal dipeptides from polypeptides. It regulates the bioactivity of many peptides, including the glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP). 50 DPP-IV inhibitors are crucial for the management of type II diabetes. This study reports for the rst time the DPP-IV inhibition potential of L. australis leaves and fruits extracts as an antidiabetic, and compared it with the control drug sitagliptin. The results (Table 3) showed that the leaves extract demonstrated the most signicant action with an IC 50 value of 90.8 ± 4.48 ng mL −1 compared to the control IC 50 = 63.8 ± 3.15 ng mL −1 . Previous reports on the antidiabetic potential of L. chinensis was evaluated by measuring the aglucosidase inhibitory effect and protein tyrosine phosphatase 1B (PTP1B) inhibitory effect, and the results showed that the butyl alcohol fraction was the most potent as an a-glucosidase and PTP1B inhibitor. 21 The activity of the leaf extract may be attributed to the avonoid content of the extract. Many avonoids reported as DPP-IV inhibitors, such as quercetin, luteolin, apigenin, kaempferol, avone, hesperetin, naringenin and genistin, in addition to glycosides as isoquercetin, kaempferol 7-O-a-L-rhamnoside, vitexin, rutin, isorhamnetin-3-O-glucoside and isorhamnetin-3-O-rutinoside, have also been reported as DPP-IV inhibitors. 8,51,52

Conclusion
This research focused on the phytochemical characterization of the leaves and fruits of Livistona chinensis and L. australis, and the biological activities of L. australis leaves and fruits. The extracts were rich with phenolic compounds, especially avonoids. Biological evaluation showed that the leaves extract displayed the best inhibitory effects against acetyl cholinesterase and DPP-IV, and good potentiation for telomerase activity. However, further experimental studies, such as in vivo animal studies and in silico study, could be planned depending on the present results.

Ethical statement
Ethics approval was not required for this research.